L3515

Sigma

 

Lectin from Artocarpus integrifolia

lyophilized powder

Synonym:Artocarpus integrifolia agglutinin, Jacalin
MDL number:MFCD00162973

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Description

Analysis NoteAgglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.
Biochem/physiol ActionsJacalin is extracted from Jack fruit seeds; a glycoprotein reported to bind human serum and secretory IgA.1 Jacalin is not blood group specific.2 The most effective inhibitor of hemagglutination is methyl α-D-galactopyranoside. The lectin is specific toward the Thomsen-Friedenreich (T) antigen, but has subtle differences in its combining site when compared to the site of Arachis hypogaea (peanut) agglutinin.3 Jacalin was also demonstrated to be a mitogen of T-lymphocytes.1
Preparation NotePurified by affinity chromatography.
Physical formContains phosphate buffer salts and NaCl

Properties

formlyophilized powder
potency<10 μg per mL agglutination activity
compositionProtein, ~70% biuret
storage temp.2-8°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3

References

Cited Reference1. Sastry, M. V., Analysis of saccharide binding to Artocarpus integrifolia lectin reveals specific recognition of T-antigen (β-D-Gal-(1→3)-D-GalNAc). J. Biol. Chem. 261, 11726, (1986)
 2. Roque-Barreira, M.C., Campos-Neto, A., Jacalin: An IgA-binding Lectin. J. Immunol. 134, 1740, (1985)
 3. Ahmed, H., Chatterjee, B.P. J. Immunol. Berlin 5, 125, (1986)